Shaqraa Musawi
Professor, University Claude Bernard (lyon1) France
Title: Role of SMN in the nucleolar reorganization after DNA repair
Biography
Biography: Shaqraa Musawi
Abstract
Most cellular transcriptional activity is carried out by the RNA polymerase I (RNAP1), which transcribe ribosomal DNA (rDNA) into ribosomal RNA (rRNA) needed in the ribosome biogenesis. During DNA Repair of UV-lesions, rDNA/RNAP1 are both reorganized within the nucleolus, namely, they migrate at the periphery of the nucleolus during DNA repair reactions and come back within the nucleolus after DNA repair completion. The proteins and exact mechanism behind these movements remain not understood. We employed various cellular and molecular biology methods, combined with confocal microscope procedures on knockdown Survival Motor Neurons (SMN) cells, and on cells of patients affected by Spinal Muscular Atrophy (SMA), to investigate whether SMN may play a role in the nucleolar reorganization during DNA repair