Rashmi Singh
University of Allahabad, India
Title: GENOTOXIC EFFECTS OF CADMIUM CHLORIDE ON PUFFING ACTIVITY OF SARCOPHAGA RUFICORNIS (FAB.) (SARCOPHAGIDAE: DIPTERA)
Biography
Biography: Rashmi Singh
Abstract
Sarcophaga ruficornis is a medically important flesh fly belonging to sarcophagidae family of Diptera, which are known to spread myiasis in cattle as well as it, has withdrawn a great attention in forensic entomology. Sarcophaga ruficornis was used cytologically to analyze polytene chromosomes from foot pad of seven day old male pupa collected from laboratory stock. Foot pads were treated with cadmium chloride for assessment of the environmental pollutants. Sarcophaga ruficornis was dissected to study the effect of cadmium chloride on polytene chromosome, which allows us to observe the mechanisms of the stress responses and the function of heat shock genes in response to thermo tolerance and adaptation to chemical stresses. Cadmium ranks close to lead and mercury in toxicological importance due to its increasing levels in the environment, as a result of industrial practices of past and present. A single puff was induced on left arm of chromosome II at the locus 12A by in vitro treatment of this heavy metal. Similar puffs have been induced by various chemical stresses (viz., heavy metals, respiratory inhibitors, hypoxia, enzymes, hormones, insecticides etc.) and heat shock effects in several sarcophagids. Thus in the present study induction of a single prominent puff is an indicator of stress response in sarcophagids, Thus it can be used as a tool providing early warning of adverse long term effects of toxic agents on the genome.